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Image Search Results
Journal: Frontiers in Pharmacology
Article Title: A Novel Agent Enhances the Chemotherapeutic Efficacy of Doxorubicin in MCF-7 Breast Cancer Cells
doi: 10.3389/fphar.2016.00249
Figure Lengend Snippet: Dox inhibited DT-010-induced GRP78 expression in MCF-7 cells. Immunoblots (A) and densitometric analysis (C) of the expression of GRP78 protein were determined after DT-010 treatment in MCF-7 cells. (B) MCF-7 cells were treated with DT-010 for 24 h in the presence or absence of Dox. Representative immunoblots for the expression of GRP78 and β-actin proteins were exhibited. (D) Densitometric analysis showed the expression of GRP78 protein. # P < 0.05 vs. Ctrl.
Article Snippet:
Techniques: Expressing, Western Blot
Journal: Frontiers in Pharmacology
Article Title: A Novel Agent Enhances the Chemotherapeutic Efficacy of Doxorubicin in MCF-7 Breast Cancer Cells
doi: 10.3389/fphar.2016.00249
Figure Lengend Snippet: Inhibition of GRP78 expression enhanced DT-010-induced cell apoptosis in MCF-7 cells. (A) The percentage of apoptotic cells in MCF-7 cells was recorded after 24 h of DT-010 treatment in the presence or absence of ER stress inhibitor 4-PBA. # P < 0.05 vs. DT-010 treated group. (B) MCF-7 cells were treated with Dox and DT-010 for 24 h, the expression of p53, cleaved-PARP, β-actin proteins were measured by western blot. The expression of GRP78 protein was determined after 12 h of Dox and DT-010 treatment. Representative immunoblots for the expression of p53, cleaved-PARP, β-actin and GRP78 proteins. (C) Densitometric quantification of the expression of GRP78 protein. (D) GRP78 knockdown further promotes DT-010-induced cell death in MCF-7 cells. ∗ means significant difference between treatments ( P < 0.05).
Article Snippet:
Techniques: Inhibition, Expressing, Western Blot, Knockdown